Molecular detection of Mycoplasma synoviae from backyard and commercial turkeys in some parts of Iran

نویسندگان

  • H. Hosseini Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
  • M. H. Bozorgmehrifard Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • N. Sheikhi Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • S. Charkhkar Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • ُS. Rasoulinezhad Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده مقاله:

M. synoviae (MS) is an economically important pathogen and the major cause of airsacculitis and infectious synovitis in turkeys. Infection with this pathogen may remain asymptomatic but can render infected birds susceptible to secondary infections. This study was carried out for the molecular detection of MS infection in commercial and backyard turkey flocks in Tehran, Semnan, Isfahan, Qazvin, Zanjan, East Azerbaijan, Gilan, Mazandaran, and Golestan provinces of Iran. Sixty-hundred tracheal, choanal cleft or/and infraorbital sinus samples were collected from 18 commercial and 31 backyard turkey flocks. The polymerase chain reaction (PCR) technique was performed by using primers specific for detecting the 16S rRNA and vlhA genes of MS. The results showed that 51.61% of backyard and 33.33% of commercial farms were MS-positive. These findings suggested the molecular presence of MS, especially in northern and central regions of Iran. Further, the frequency of MS-positive samples was significantly lower in commercial farms than backyard farms (P

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عنوان ژورنال

دوره 73  شماره 2

صفحات  79- 85

تاریخ انتشار 2018-06-01

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